The gel ran for too long. Some of the smaller pieces are missing. Some of these could be determined from subtraction.I have given the missing bands in the comments.
The band in every lane that runs around 4800 is uncut coiled plasmid. Do not quantify this band in any of your analysis.
Lane Number
Contents
Missing bands that ran off the end
1
EcoRI
2
HincII
3
RsaI
680
4
Ladder
5
HincII+RsaI
There are two bands that are close together. missing are: 619, 488, 61
6
EcoRI+HincII
655, 452
7
EcoRI+RsaI
513, 167
8
EcoRI+AvaI
9
AvaI+RsaI
856 is faint, 680 is missing
10
AvaI+HincII
The faint one is 772
Gel image (Right-click to download full-size file):
The band in every lane that runs around 4800 is uncut coiled plasmid. Do not quantify this band in any of your analysis.